Construction of the prokaryotic expression vector of NS5ATP4A gene and its expression

Objective To construct prokaryotic expression vector of NS5ATP4A gene and express it in E.coli.Methods The NS5ATP4A gene was amplified by PCR and cloned in to plasmid pET32a(+) to construct recombinant prokaryotic expression vector pET32a(+)-NS5ATP4A.After transfection into the E.coli and induction with IPTG,recombinant target protein was analyzed by SDS-PAGE and approved by Western Blot.Results The recombinant prokaryotic expression vector was constructed successfully.The antigenicity of 35KD target protein,induced by IPTG was proved by Western Blot.Conclusion The construction of prokaryotic expression vector pET32a(+)-NS5ATP4A provides the foundation for the preparation of antibody in clinical test.