Curcumin-induced apoptosis of human liver cancer Bel-7402 cell line

Objective To investigate the apoptosis of human liver cancer Bel-7402 cell line induced by curcumin in vetro.Methods The cells were treated in vetro with curcumin 10μmol/L for 48h.Inhibiting effect of cell proliferation was detected with MTT assay,cell apoptosis was analyzed by flowcytometry,and the expressions of Bcl-2 and Bax mRNA were detected by RT-PCR.ResultsAfter treated by curcumin for 48 h,the cell proliferation was inhibited in time-and dose-dependent manner.As the concentration of curcumin increased,the apoptosis rate of the cells increased,expression of Bax mRNA up-regulated and that of Bcl-2 mRNA down-regulated.ConclusionCurcumin inhibits cell proliferation and enhances cell apoptosis,which may be related to up-regulating Bax mRNA expression and down-regulating Bcl-2 mRNA expression.