The Detection of Plant Glutathione Peroxidase Gene Expression by Blot Hybridization with Digoxigenin-labelled cDNA Probe and Method Research

In this work,ATGPX3 cDNA probe labelled with digoxigenin was prepared by using RT-PCR and PCR methods.A series of DNA and RNA were detected by dot blot hybridization analysis.In order to improve hybridization reaction,the paper changed concentrations of some constituents of hybridization buffer and added 10% Dextran Sulphate.The results showed that the improved protocol not only raised the hybridization efficiency,but also obviously detected signal by RNA blot hybridization analysis.Moreover,ABA-induced ATGPX3 gene expression was also detected using digoxigenin-labelled cDNA probe,and this method could be used to detect the gene expression in plants.