Shi‐Wen Jiang, S Zhang
Abstract
A simple, rapid method for colony hybridization has been developed. The DNA probes were labelled by digoxigenin. Hybridization signal was detected by enzyme-linked immunoassay reaction. The results showed that this method is sensitive and reproducible, and it can be used for colony hybridization instead of isotopic methods.
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A simple, rapid method for colony hybridization has been developed. The DNA probes were labelled by digoxigenin. Hybridization signal was detected by enzyme-linked immunoassay reaction. The results showed that this method is sensitive and reproducible, and it can be used for colony hybridization instead of isotopic methods.
Key concepts: Digoxigenin, Hybridization probe, Molecular biology, DNA–DNA hybridization, Biology, Nucleic acid thermodynamics, In situ hybridization, DNA