2010•Zhongguo yaofangRequires access

Antioxidant Activity of Polygonum capitatum

Yan Xing-lian, Wenyi Kang

Abstract

OBJECTIVE:To investigate the antioxidant activity of Polygonum capitatum.METHODS:The total antioxidant activity of P.capitatum was assayed by three methods,such as 1,1-diphenyl-2-picryl-2-picrylhydrazyl(DPPH) radical scavenging,[2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid] diammonium salt(ABTS) radical scavenging,and ferric reducing antioxi-dant power(FRAP) assay.The results were compared with 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid(Trolox) and positive control butylated hydroxyanisole(BHA),2,6-di-tert-butyl-4-methylphenol(BHT).RESULTS:The results showed that P.capitatum had good antioxidant activity in vitro.The methanol extract showed higher scavenging activity against DPPH radical,ABTS radical,and reducing antioxidant power of TPTZ,and the methanol extract had higher total antioxidant activity than that of BHT.CONCLUSION:Among three kinds of extracts from P.capitatum,the methanol extract shows the highest antioxidant activity,followed by ethyl acetate extract.The results of DPPH are correlated with that of ABTS assay(r=0.991 7).

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Key concepts: Chemistry, Butylated hydroxyanisole, DPPH, Trolox, ABTS, Antioxidant, Methanol, Organic chemistry