Study on conditions of the enzyme hydration reaction

Taking the enzyme of free cell as the catalyst,the effects of dilution factor,concentration of acrylonitrile,temperature,pH value,concentration of cells and concentration of acrylmide on the activity of nitrile hydratase were studied.The result shows that the temperature,the concentration of acrylonitrile and the concentration of acrylamide are the most important among these factors.The activity of nitrile hydratase is 4 901U/mL(broth) at the reaction temperature 28℃;the concentration of acrylonitrile is 40g/L;the activity of nitrile hydratase in the solution of 40% acrylamide is just 40% of that in solution of 5% acrylamide.It is found that the activation energy of the nitrile hydratase is 40.291kJ·mol-1through the research at the different reaction temperature.