Dynamic observation on the intraperitoneal glucose tolerance test in the non-hyperglycemic rats treated with streptozotocin

Objective:To study whether the glucose tolerance was impaired in the non-hyperglycemic rats treated with strepto-zotocin(STZ).Methods:Male SD rats,aged 10￣12 weeks,were divided into two groups:experimental group,with an intraperitoneal injection of 30 mg/kg(low dose)or 60 mg/kg(high dose)STZ;control group,with injection of equal dose acidified saline.1￣12 weeks after injection,the intraperitoneal glucose tolerance tes(tIPGTT)was performed in the non-hyperglycemic rats(fasting blood glucose 10 mmol/L)treated with STZ.Results:There was no significant difference in the fasting blood glucose or IPGTT between the control and the low dose groups.2/9￣3/5 of the non-hyperglycemic rats treated with high dose STZ had abnormal IPGTT,i.e,at 120 min after intraperitoneal injection of 50% glucose(2 g/kg),the fasting blood glucose levels were high-er than those in all the animals in the corresponding control group,and≥10 mmol/L.Conclusion:Low dose STZ did not in-duce hyperglycemia or abnormal IPGTT in all rats,and the non-hyperglycemic rats treated with high dose STZ had an impaired glucose tolerance.