Optimization ultrasonic extraction process of total saponins from Asparagus officinalis L. by response surface methodology

To optimize the ultrasonic extraction technique for the total saponins from Asparagus officinalis L. by response surface methodology. The effect factors on the yield of total saponins from Asparagus officinalis L.were studied with ultrasonic condition,including ethanol concentration,solid-liquid ratio,ultrasonic time,ultrasonic temperature. The optimized extraction process was found through the response surface methodology. According to the principle of central composite design with the response surface methodology of three factors and three levels,the optimum ultrasound extraction technology was as following:solid-liquid ratio1∶15(W/V),74% ethanol,ultrasonic time 54 min,ultrasonic temperature 50℃. Under the conditions,the extraction yield of the total saponins of Asparagus officinalis L. was 13.06%,which was well matched with the predictive extraction yield 13.18%.