Effects of Celecoxib on Expression of Bcl-2 and Bax Protein in Neural Cells of Mouse Brain Following Pentylenetetrazole-kindled Epilepsy

Objective:To explore the effects of celecoxib on the epileptic seizures and expression of Bcl-2 and Bax protein in neural cells of mouse brain following pentylenetetrazole-kindled epilepsy. Methods: Thirty C57BL/6 mice were randomly divided into control group (n=10),epilepsy group (n=10) and celecoxib treatment group (n=10). The effects of celecoxib on epileptic status in mice in different groups were investigated and the expression of Bcl-2 and Bax protein in brain cells of the mice were examined. Results: Relative to those in the control group,the Bcl-2 and Bax positive neural cells were significantly increased and the ratio of Bcl-2/Bax was significantly decreased in mice in the epilepsy group (P0.01). The Bax positive neural cells was significantly decreased whereas the Bcl-2 positive neural cells and the ratio of Bcl-2/Bax were significantly increased in mice in the celecoxib treatment group when compared to those in the mice in the epilepsy group (P0.01). Conclusion: Treating with celecoxib can down-regulate the expression of Bax protein,up-regulate the expression of Bcl-2 protein and increase the ratio of Bcl-2/Bax in brain of the mice following pentylenetetrazole-kindled epilepsy.