Cloning of Two Genomics Genes and Sequence Analysis Encoding Calmodulin from Peanut (Arachis hypogaea L.)

Two heteromotphic CaM genes in genomics of peanut(Arachis hypogaea L.) leaflet were cloned by PCR reaction using primers synthesized accord to the CaM conservative sequences and registered in Genebank as AY517933 and AY572856 respectively.Result showed that the whole length of PGCaM-1 gene was 1425 base pairs and had a 973 base pairs intron behind the 25th amino acid while PGCaM-3 gene was 447 base pairs.Both of their open reading frame were composed of 447 base pairs encoding 148 amino acids and sequence identity between them were 96% and 98% respectively.In addition, they both belonged to the EF-Hand super-family of calmodulin and also shared high homology with known peanut calmodulin cDNA sequence and other higher plants,The title differences maybe result in different expressions and functions.