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Codon Optimization and Expression of MAP30 in Pichia pastoris Expression System

Fan Wang

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Abstract

Taking Momordica charantia as experimental material,the codon optimized MAP30gene of Momordica charantiain Pichia pastoris expression was studied by SDS-PAGE and Western-blot.The results showed that the expression quantity of the optimized MAP30gene was increased slightly in the neotype Pichia pastoris expression system.pGAPHαvector composed of GAP promotor was safer than pPIC9composed of AOX promotor.It is supposed that the new way should be used in medical field.

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What this paper is about

Taking Momordica charantia as experimental material,the codon optimized MAP30gene of Momordica charantiain Pichia pastoris expression was studied by SDS-PAGE and Western-blot.The results showed that the expression quantity of the optimized MAP30gene was increased slightly in the neotype Pichia pastoris expression system.pGAPHαvector composed of GAP promotor was safer than pPIC9composed of AOX promotor.It is supposed that the new way should be used in medical field.

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Available abstract

Taking Momordica charantia as experimental material,the codon optimized MAP30gene of Momordica charantiain Pichia pastoris expression was studied by SDS-PAGE and Western-blot.The results showed that the expression quantity of the optimized MAP30gene was increased slightly in the neotype Pichia pastoris expression system.pGAPHαvector composed of GAP promotor was safer than pPIC9composed of AOX promotor.It is supposed that the new way should be used in medical field.

Key concepts: Pichia pastoris, Momordica, Expression vector, Promoter, Pichia, Electroporation, Biology, Molecular biology

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