Identification MRC-5 cell by using silver staining PCR product from STR system

Silver staining of ten-locus(nine STR loci and gender identification locus) system was used to identify Master Cell Bank,Working Cell Bank and the limited passages of MRC-5 cell line.Nine STR loci of MRC-5 and Amelogenin locus(the gender identification locus) were amplified by PCR and separated with a denatured PAGE electrophoresis followed by the silver staining.Compared to the eight fluorescence staning STR loci and the Amelogenin locus of MRC-5 published by ATCC,eight overlapped loci(CSF1PO,TPOX,TH01,FESFPS,vWA,D16S539,D7S820,D13S317 and Amelogenin) are comformable.And the identity of the three passages of MRC-5 was confirmed too.Therefore silver staining STR system is a simple,practical and exact method in identification of cell lines in a broad range.