The practical use of fluorescence-based STR typing technique for decomposed tissues

To detect STR genotypes of degraded DNA from decomposed tissues. The PCR apmlified products of 40 degraded DNA samples, which were purified from decomposed muscles after death for 1～6 weeks, were separated by denaturing polyacrylamide. The genotypes were analyzed by 377 DNA sequencer and 2 fluorescence-based multiplex amplification systems which contain 12 STR loci and lsex locus. The specific PCR products of 12 STR loci and 1 sex locus of the 40 samples were typed precisely and rapidly. The method described here is very useful in forensic case works, especially for the seriously degraded DNA samples.