THE COMPARISON OF THE EXPRESSIONS OF p16~(ink4a) AND Ki-67 IN HUMAN CERVICAL LESIONS

Objective To compare the expressions of p16~(ink4a) and Ki-67 in human cervical lesions. Methods The expressions of p16~ink4a and Ki-67 protein were measured with immunhistochemical staining in 42 human normal uterine cervical tissues,21 with human cervical intraepitheial neoplasia(CIN) Ⅰ,21 with CINⅡ,and 36 with CINⅢ.The relevance of the expressions of the two proteins to CIN grades was analyzed by Spearman Correlation.The sensitivity,specificity,positive/negative predictive values,and youden's index of p16~(ink4a) and Ki-67 were also studied. Results For p16~(ink4a),the sensitivity and specificity were 83.3% and 90.5%;the positive and negative predictive values were 94.2% and 74.5%;Youden's index was 0.738.For ki-67,the sensitivity and specificity were 98.7% and 16.7%;the positive and negative predictive values were 68.75% and 87.5%;Youden's index was 0.154.Both the sensitivity and specificity for the combined Ki-67 and p16~(ink4a) are 83.3%.Conclusion p16~(ink4a) has higher specificity and positive predictive value,and is a good diagnostic marker for cervical lesion.By contrast,Ki-67 has higher sensitivity and negative predictive value.Even with its poor specificity,it can still be regarded as a supplementary test for early diagnostic cervical lesion.The overall performance of p16~(ink4a) is better than that of Ki-67.