Preparation of Reducing Sugar Using Ultrasonic Pretreatment and Aspergillus Niger Synergistic Hydrolysis for Corncob

Reducing sugars was made from ultrasonic pretreatment and aspergillus niger synergistic hydrolysis for corncob.The effects of sample size,volume of the enzymolysis solution,reaction time and reaction temperature on reducing sugar yield were investigated.The reaction condition such as temperatures,times and volume of the enzymolysis solution were analyzed by response surface methodology(RSM),and got the best processes.The result showed that under a reaction temperature of 56 ℃,a reaction time of 22.4 h,and a volume of the enzymolysis solution of 26 U/g,the reducing sugar could reach 36.58 %.The reducing sugar yield had been increased 13.41 % compared with the sample which hadn't pretreat with ultrasonic microwave at the same condition.