Colchicines-induced polyploid plants and identification in Lilium pumilum DC.

This work was aimed to obtain tetraploid Lilium pumilum DC with excellent ornamental traits and agronomic characters,and provide better cross parent for breeding of new Lilium variety.The seeds of Lilium pumilum DC(2n=2x=24) were treated with different concentrations of colchicine solution at different hours in order to figure out a proper method and obtain polyploids.The results indicated that the most effective induction was obtained by 0.1 % colchicine treatment for 24 hours with 30 % mutation rate.By chromosome observation of root tip,the tetraploid plants were successfully obtained with the chromosome number 2n=4x=48.The tetraploid plants showed obvious characteristics of low stoma density,large stoma,slow growth,thick and wide leaves and dark leaf color at the initial stage,but quick growth and large leaves at later stage.The results indicated that the polyploid plants were identified by phenotype,stomas and chromosome number observation.