Construction and identification of mouse integrin β_1 shRNA expression vector

Objective To construct mouse integrin β1 shRNA expression vector,and to down-regulate the expression of integrin β1 in mouse airway smooth muscle cells by RNA interference.Methods The shRNA expression vector targeting mouse integrin β1 gene was designed and constructed to transfect the airway smooth muscle cells,and detect the inhibiting effect of shRNA expression vector on the targeted genes.Results The shRNA expression vector can stably transfect the mouse airway smooth muscle cells.The expression of mRNA and protein of integrin β1 was down-regulated after transfection.Conclusion The shRNA expression vector targeting mouse integrin β1 gene was successfully constructed,which can down-regulate the expression of integrin β1 after transfection.This study facilitates further studies on the function of integrin β1 in the airway remodeling of asthma and its application in asthmatic gene therapy.