Construction of integrin β_1 shRNA expression plasmid and its regulation on proliferation in cell line SMMC-7721

Objective To construct integrinβ1 subunit small interfering RNAs expression plasmid and to observe its influence on the proliferation in human cell line SMMC-7721.Methods Using RNA interference techniques,a DNA vector-driven shRNAs expression plasmid was constructed with pSilencer2.0 vector containing U6 promotor.After sequencing,this plasmid was transfected into SMMC-7721.Then the transfected cells were harvested,and the expression of integrinβ1 and p27 protein were detected by western blot.Meanwhile,the changes of cell proliferation were analyzed by MTT assay.Results The integrinβ1 shRNA expression plasmid was constructed successfully.After plasmid transfection,the protein amount of both integrin β1 and p27 were reduced.Moreover,MTT assay indicated that the proliferation ability of transfected cells was enhanced compared with the negative control group.Conclusion shRNA expression plasmid could specifically inhibit the protein expression of integrinβ1.Integrinβ1 could regulate the cell growth in SMMC-7721,and p27 may play an important role herein.