Effects of medium components on growth of mycelium of Cordyceps militaris and medium optimization

Using mycelium biomass as evaluation parameter,effects of medium components on growth of Cordyceps militans were investigated.The critical components were screened with Plackett-Buman experimental design and the optimum composition of medium was determined with the steepest ascent method and Center Composite Design(CCD).The results showed that glucose,KH_2PO_4 and yeast extract had the most significant effects on mycelium biomass.The optimum medium for growth of mycelium were as follows,59.35g/L glucose,16.83g/L yeast extract,1.47g/L KH_2PO_4,10g/L peptone and 0.1g/L MgSO_4·7H_2O.By optimization of culture conditions,the Yield of mycelium biomass reached 26.86g/L,which was 2.5 times as that of original medium,when optimum medium was used.It is indicated that application of optimized medium had much potential for improved mycelium biomass of C.militaris in industry.