Optimization of Extraction Process for Anti Hepatitis B Consituents from Acid Litchi Semen by Response Surface Methodology

Objective: To optimize extracting process of total flavonoids from acid Litchi Semen.Method: Taking rutin as standard,the content of total flavonoids was determined by UV. With yield of total flavonoids as index,on the basis of single factor tests,Box-Behnken and Plackett-Burman tests were adopted to optimize extraction process by taking temperature,grinding degree,ethanol concentration,liquid-solid ratio,extracting time and stirring speed as factors. Result: Grinding degree and ethanol concentration were significant factors,optimum extraction technology was as following: grinding degree of 120 mesh,extracted 2 h with 14 times the amount of 75% ethanol at 60 ℃,stirring speed of 90 r·min- 1. Under these conditions,average extraction rate of total flavonoids was 17. 49% with RSD of 0. 63%. Conclusion: This optimized technology is stable and feasible,which is suitable for industrial production of anti-hepatitis B flavonoids from acid Litchi Semen.