Therapeutic Effect of Curcumin Combined with Mesalazine on Ulcerative Colitis in Mice

Objective To investigate the effect of curcumin combined with mesalazine on the treatment of ulcerative colitis in mice and its mechanism.Methods Fifty mice were divided into 5 groups:normal group,model group,curcumin combined with mesalazine treatment group,curcumin treatment group and mesalazine treatment group.The mouse models with ulcerative colitis were copied by dextran sulfate so-dium traditional method.Since the model was successfully constructed,the next day,curcumin combined received mesalazine treatment group received curcumin enema treatment and mesalazine orally fed of each;curcumin treatment group received curcumin enema treatment;mesalazine treatment group received mesalazine orally fed;normal group and model group were treated with correspondingly 9 g·L-1 saline.All once every day,a total for 10 d.Ten days after administration,the pathological changes were observed,and flow cytometry was used to detect the apo-ptosis of colonic epithelial cells,ELISA was used to detect the serum IL-2 and IL-10 levels.Results The colonic damage scores,histolo-gical scores and the apoptosis rate in curcumin combined with mesalazine treatment group were significantly lower than those in model group(Pa0.01).Compared with the curcumin treatment group and mesalazine treatment group,the injury score,histological score and the apoptosis rate in curcumin combined with mesalazin treatment group decreased,and the differences were statistically significant(Pa0.05).Compared with model group,IL-10 levels decreased and IL-2 levels increased significantly in curcumin combined with mesalazine treatment group and the curcumin treatment group and mesalazine treatment group(Pa0.01).Compared with the curcurnin treatment group and mesalazine treatment group,IL-10 levels decreased and IL-2 levels increased significantly in curcumin combined with mesalazine treatment group(Pa0.05).Conclusion Curcumin combined with mesalazine therapy is better than using mesalazine alone on mice with ulcerative colitis,and also effective in decreasing IL-10 level and increasing IL-2 level compared with using mesalazine alone.