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2003•Xiandai linchuang yixue shengwu gongchengxue zazhiRequires access

Construction of an Eukaryotic Expression Vector Inserted HBsAg Gene

Du Zhi

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Abstract

Objective To construct a recombinant eukaryotic expression plasmid inserted HBsAg gene.Methods The full length gene of HBsAg from plasmid pEcob6 was amplified by PCR. Then the PCR product was digested with HindⅢ and BamHⅠ.Finally, the PCR product was cloned into plasmid pcDNA3.1(+).The accuracy of pcDNA3.1-S was confirmed by restriction enzyme digestion and DNA sequencing.Results Restriction enzyme digestion and DNA sequencing confirmed that the recombinant eukaryotic expression plasmid inserted HBsAg gene (pcDNA3.1-S) had been constructed correctly.Conclusions An eukaryotic expression plasmid pcDNA3.1- S has been constructed successfully.

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What this paper is about

Objective To construct a recombinant eukaryotic expression plasmid inserted HBsAg gene.Methods The full length gene of HBsAg from plasmid pEcob6 was amplified by PCR. Then the PCR product was digested with HindⅢ and BamHⅠ.Finally, the PCR product was cloned into plasmid pcDNA3.1(+).The accuracy of pcDNA3.1-S was confirmed by restriction enzyme digestion and DNA sequencing.Results Restriction enzyme digestion and DNA sequencing confirmed that the recombinant eukaryotic expression plasmid inserted HBsAg gene (pcDNA3.1-S) had been constructed correctly.Conclusions An eukaryotic expression plasmid pcDNA3.1- S has been constructed successfully.

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Available abstract

Objective To construct a recombinant eukaryotic expression plasmid inserted HBsAg gene.Methods The full length gene of HBsAg from plasmid pEcob6 was amplified by PCR. Then the PCR product was digested with HindⅢ and BamHⅠ.Finally, the PCR product was cloned into plasmid pcDNA3.1(+).The accuracy of pcDNA3.1-S was confirmed by restriction enzyme digestion and DNA sequencing.Results Restriction enzyme digestion and DNA sequencing confirmed that the recombinant eukaryotic expression plasmid inserted HBsAg gene (pcDNA3.1-S) had been constructed correctly.Conclusions An eukaryotic expression plasmid pcDNA3.1- S has been constructed successfully.

Key concepts: Plasmid, Molecular biology, Recombinant DNA, Restriction enzyme, HBsAg, Gene, Vector (molecular biology), Biology

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