The biological study on the components of erythrocyte membrane of the qi depression to blood stasis rats

Objective To investigate biological difference of hemorheology of pneuma depression and blood stasis from molecular level of red cell membrane. Methods Twenty wistar rats were randomly assigned to blank control group and pneuma depression and blood stasis group, with 10 rats in each group. The related indexes of hemorheology and the components of erythrocyte membrane were determined after molding 15 days. Results Compared with blank control group, the weight of pneuma depression and blood stasis group was decreased significantly. There was no significant change in the whole blood viscosity, plasma viscosity and the red cell deformability index. All of them were decreased. APTT was curtailing significantly (P 0.01) while there were no significant change in PT, TT and FIB. Na+ -K + -ATP enzyme and SOD activity of erythrocyte membrane are decreasing (P 0.01, P 0.05). The content of sialic acid in erythrocyte membrane was decreased significantly (P 0.05) while MDA was increased significantly (P 0.05). Conclusion The pneuma depression and blood stasis model can decrease the content of sialic acid and SOD and Na+ -K+ -ATP enzyme activity of erythrocyte membrane. Maybe strong and short time stimulation in experiment cause that there is no significant change in the whole blood viscosity and the red cell deformability. Maybe there is some relationship with clotting mechanism which include APTT. This model should be studied further.