Effects of pyrrolidine dithiocarbamate on aquaporin-9 expression and brain edema around the hematoma in experimental intracerebral hemorrhage

Objective To investigate the effects of pyrrolidine dithiocarbamate(PDTC) on aquaporin-9(AQP9) expression and brain edema around the hematoma in experimental intracerebral hemorrhage(ICH).Methods 128 SD rats were randomly divided into four groups: normal group,control group,ICH group and PDTC group.According to Rosenberg's methods,the models of experimental ICH were established.The rats in PDTC group were received PDTC intraperitoneally 2 h later.The brain weight column(BWC) at different time point was calculated by gray-wet method,and the expression of AQP9 was examined by immunohistochemistry.Results The BWC and expression of AQP9 in hematoma side of ICH begin to increase 4 h after ICH,peaked at 72 h,and decreased 120 h later.The BWC in opposite side of hematoma also increased 4 h after ICH and peaked at 72 h.Compared with control group,the BWC and expression of AQP9 in ICH group were higher,especially in hematoma side of brain(all(P)0.01).Compared with ICH group,the BWC and expression of AQP9 in PDTC group significantly decreased 24 h after ICH(all(P)0.01).Conclusions BWC and the expression AQP9 increase at the same time after ICH and may be inhibited by intervention of PDTC.AQP9 is involved in the brain edema after ICH.