2011•Journal of Xinxiang UniversityRequires access

Secretion Expression of Mature Mouse Liver-expressed Antimicrobial Peptide-2 in Pichia Pastoris

Hongliang Wang

Abstract

The pPIC9-mmLEAP-2 expression vector was transformed into Pichia pastoris GS115,the transform with multi-copy inserts were selected by PCR identification,the expression of recombinant mmLEAP-2 induced by methanol was analyzed by SDS-PAGE,which provides a basis for further research on antibacterial activity of mmLEAP-2.

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Key concepts: Pichia pastoris, Recombinant DNA, Pichia, Antimicrobial, Secretion, Expression vector, Antimicrobial peptides, Biology