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2011•Journal of Inner Mongolia University for the NationalitiesRequires access

Construction and Identification of Expression System of rAp-B in Pichia pastoris

Cheng Yan

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Abstract

Synthesis of Ap-B gene sequence which was inserted into pPICZαvector was designed by code-modifications of pastoris.Transformed pPICZαvector into Pichia pastoris X33 via electroporation.Then Expression System was Identificated by PCR.The transformed Pichia pastoris screened by using the expression primers.The resurt show that our studies succeed in secreted expression system of rAp-B in Pichia pastoris and 4 strains of engineered bacteria identified.

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What this paper is about

Synthesis of Ap-B gene sequence which was inserted into pPICZαvector was designed by code-modifications of pastoris.Transformed pPICZαvector into Pichia pastoris X33 via electroporation.Then Expression System was Identificated by PCR.The transformed Pichia pastoris screened by using the expression primers.The resurt show that our studies succeed in secreted expression system of rAp-B in Pichia pastoris and 4 strains of engineered bacteria identified.

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Available abstract

Synthesis of Ap-B gene sequence which was inserted into pPICZαvector was designed by code-modifications of pastoris.Transformed pPICZαvector into Pichia pastoris X33 via electroporation.Then Expression System was Identificated by PCR.The transformed Pichia pastoris screened by using the expression primers.The resurt show that our studies succeed in secreted expression system of rAp-B in Pichia pastoris and 4 strains of engineered bacteria identified.

Key concepts: Pichia pastoris, Electroporation, Expression vector, Pichia, Molecular biology, Vector (molecular biology), Biology, Gene

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