2005•Liaoning zhongyi zazhiRequires access
Effect of Sanshengyin on the Expression of Bcl-2 and Bax Protein in the Rats after Focal Cerebral Ischemia
Yuanyuan Wang
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Abstract
Objective :To study the effect of Sanshengyin on the expression of Bcl 2 and Bax protein in the rats after focal cerebral ischemia. Methods :Creation the rat model of middle cerebral artery occlusion (MCAO) and reperfusion.Using the tetraphenyl tetrazolium chloride(TTC) staining detected the ischemic zone,Immunohistochemistry method analyze the expression of Bcl 2 and Bax protein positive cells at 3h,6h,12h,24h,3d after reperfusion.Compared the results between the group of MCAO and Sanshengyin. Results :Compared with the results of the MCAO group,Sanshengyin could relieved the lesion of cerebral ischemia;reduced the size of the infarct;and in this group,Bcl 2 protein positive cells increased obviously.But Bax protein positive cells did not decrease,the results of the two group have no significant differentiation( P 0 05). Conclusion :Sanshengyin is the effective neuroprotective by increasing the expression of Bcl 2 protein,when the cerebral ischemia reperfusion injury occurring.