The Effects of Paclitaxel on the Plasma Level of Catecholamine and Some Immune Parameters during Perioperative Period in Rat Model of Breast Cancer

Objective:To investigate the effects of paclitaxel on the plasma level of catecholamine and some immune parameters during the perioperative period in rat model of breast cancer.Methods:Seventy SD rats were used to establish the model of breast cancer.Fifty rats,successfully formed tumor in the left armpit by planting walker-256 cells,were divided into 4 experimental groups and 1 control group randomly(n=10 for each group).Rats of experimental groups were received intravenous infusion of paclitaxel(25 μg/g),while rats in control group was given the same dose of normal saline.The tumor resection operation removal surgery was performed after 1,2,3 and 4w treatment in rats of experimental groups.The blood levels of epinephrine,norepinephrine,natural killer(NK) cell count,CD4+/CD8+ ratio,tumor necrosis factors-α(TNF-α) and interleukine-6(IL-6) were detected before and after operation in rats of groups.Results:There were no significant differences in epinephrine,TNF-α and NK cell counter before and after the operation in rats of five groups(P 0.05).But the level of norepinephrine was higher before and after operation in group 1 and group 2 than that of control group.The level of CD4+/CD8+ was lower before and after operation in group 1 and group 2 than that of control group.The ratio of CD4+/CD8+ was lower in group 3 than that in control group.The levels of IL-6 were significantly lower before and after operation in groups 1,2 and 3 than those of control group(P 0.01).Conclusion:Intravenous infusion of paclitaxel can induce significant stress response characterized by the increasing of plasma level of norepinephrine,and also can suppress the immune system.