Optimization for Extracting Total Flavonoids from Hypericum ascyron L. by Enzyme Assisted Method

The extracting process of the total flavonoids from Hypericum ascyron L. by enzyme assisted method was opti- mized. On the basis of single factor tests and orthogonal experiment, the effects of enzymatic treatment temperature, pH val- ue, enzyme dosage, and enzymatic treatment time on the total flavonoids content were studied. The results showed that the optimal enzymatic extracting process of total flavonoids were enzyme dosage 1.5%, enzymatic temperature 35 ℃, enzymatic time 1.0 h, and pH value 5.2, and the total flavonoids content reached 13.26 mg / g, 10.2% more than that by ultrasonic extraction method.