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STUDY ON THE EXTRACTION OF TOTAL FLAVONOIDS FROM CHINESE PINE POLLEN BY ENZYMATIC

Treatment Yang

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Abstract

A new technology of extracting total flavonoids from Chinese pine pollen by enzymatic treatment was studied.NaNO2-Al(NO3)3-NaOH colorimetric method was applied to determine the total flavonoids. The optimum technological conditions were followed:the concentration of enzyme was 0.35 g/L,enzymatic hydrolysis time was 150 min,enzymatic hydrolysis temperature was 55 ℃,pH of extracting media is 5.0. The content of flavonoids the extraction liquid was as high as 1.55 %.

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A new technology of extracting total flavonoids from Chinese pine pollen by enzymatic treatment was studied.NaNO2-Al(NO3)3-NaOH colorimetric method was applied to determine the total flavonoids. The optimum technological conditions were followed:the concentration of enzyme was 0.35 g/L,enzymatic hydrolysis time was 150 min,enzymatic hydrolysis temperature was 55 ℃,pH of extracting media is 5.0. The content of flavonoids the extraction liquid was as high as 1.55 %.

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Available abstract

A new technology of extracting total flavonoids from Chinese pine pollen by enzymatic treatment was studied.NaNO2-Al(NO3)3-NaOH colorimetric method was applied to determine the total flavonoids. The optimum technological conditions were followed:the concentration of enzyme was 0.35 g/L,enzymatic hydrolysis time was 150 min,enzymatic hydrolysis temperature was 55 ℃,pH of extracting media is 5.0. The content of flavonoids the extraction liquid was as high as 1.55 %.

Key concepts: Enzymatic hydrolysis, Extraction (chemistry), Chemistry, Chromatography, Enzyme, Hydrolysis, Pollen, Botany

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