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2010•Zhongliu fangzhi yanjiuOpen access

Expression and Significance of FoxO3a in MCF-7 Cells Apoptosis Induced by Arsenic Trioxide

Hong Liu

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Abstract

Objective To investigate the apoptosis of MCF-7 cells induced by different concentrations of As2O3 and expressions of FoxO3a and Caspase-3 protein.Metheds The apoptosis of human breast carcinoma MCF-7 cells treated for 24 hours with 2.0,4.0,8.0 μmol/L As2O3 respectively was detected by flow cytometry.The activities of FoxO3a and Caspase-3 proteins were examined by immunocytochemistry and Western blot in untreated or treated by As2O3.Results The rate of the apoptosis in MCF-7 cells treated by 2.0,4.0,8.0 μmol/L of As2O3 were(6.26±0.94)%,(9.30±1.63)% and(13.02±3.82)%,respectively.There were obvious dose-effect correlations between As2O3 and apoptosis(rs=0.949,P0.01).As2O3 promoted the expression of nucleus-FoxO3a and Caspase-3 protein.Conclusion The results suggested that As2O3 could induce apoptosis of MCF-7 cells via FoxO3a protein activation and Caspase-3 protein up-regulation.

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Objective To investigate the apoptosis of MCF-7 cells induced by different concentrations of As2O3 and expressions of FoxO3a and Caspase-3 protein.Metheds The apoptosis of human breast carcinoma MCF-7 cells treated for 24 hours with 2.0,4.0,8.0 μmol/L As2O3 respectively was detected by flow cytometry.The activities of FoxO3a and Caspase-3 proteins were examined by immunocytochemistry and Western blot in untreated or treated by As2O3.Results The rate of the apoptosis in MCF-7 cells treated by 2.0,4.0,8.0 μmol/L of As2O3 were(6.26±0.94)%,(9.30±1.63)% and(13.02±3.82)%,respectively.There were obvious dose-effect correlations between As2O3 and apoptosis(rs=0.949,P0.01).As2O3 promoted the expression of nucleus-FoxO3a and Caspase-3 protein.Conclusion The results suggested that As2O3 could induce apoptosis of MCF-7 cells via FoxO3a protein activation and Caspase-3 protein up-regulation.

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Available abstract

Objective To investigate the apoptosis of MCF-7 cells induced by different concentrations of As2O3 and expressions of FoxO3a and Caspase-3 protein.Metheds The apoptosis of human breast carcinoma MCF-7 cells treated for 24 hours with 2.0,4.0,8.0 μmol/L As2O3 respectively was detected by flow cytometry.The activities of FoxO3a and Caspase-3 proteins were examined by immunocytochemistry and Western blot in untreated or treated by As2O3.Results The rate of the apoptosis in MCF-7 cells treated by 2.0,4.0,8.0 μmol/L of As2O3 were(6.26±0.94)%,(9.30±1.63)% and(13.02±3.82)%,respectively.There were obvious dose-effect correlations between As2O3 and apoptosis(rs=0.949,P0.01).As2O3 promoted the expression of nucleus-FoxO3a and Caspase-3 protein.Conclusion The results suggested that As2O3 could induce apoptosis of MCF-7 cells via FoxO3a protein activation and Caspase-3 protein up-regulation.

Key concepts: Arsenic trioxide, MCF-7, Apoptosis, Immunocytochemistry, Molecular biology, Chemistry, Flow cytometry, Western blot

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