Research of the inhibitory mechanism of arsenic trioxide on human amalignant melanoma A_(375) cells

Objective:To investigate the growth inhibition role of arsenic trioxide(As2O3) on human amalignant melanoma A375 cell,and observe whether Caspase-3 protein can be expressed in A375 cell after induced by As2O3.To provide theory and experiment base for treament of malignant melanoma. Methods:The morphologic changes of A375 cells were detected by fluorescence staining,apoptosis rate of A375 cells was detected with flow cytometry.Caspase-3 protein was detected by immunehistochemistry in A375 cells.The expression of Survivin mRNA was examined by semi-quantitative RT-PCR. Results:As2O3 caused morphologic change of A375 cells by fluorescence staining.As2O3 promoted A375 cell apoptosis significantly.When A375 cells were treated with As2O3 in 5,10,and 20 μmol/L for 24 hours,the apoptosis rate was 7.07%,38.66% and 4.34%.Caspase-3 protein was expressed positively in A375 cells by co-culture with As2O3.The expression of Survivin mRNA was inhibited by As2O3. Conclusions:As2O3 can cause morphologic change of A375 cells and promote apoptosis happening.As2O3 can induce A375 cells to express Caspase-3 protein.As2O3 can inhibit Survivin protein expression in A375 cells.