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Cloning of 5 flanking sequence of goat BLG and regulating the expression of GFP in cell

Song Hong

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Abstract

′flanking region of goat BLG was amplified from goat genomic DNA according to the published whole sequence of goat BLG and cloned to pMD 18t vector correspondently.By sequencing,the amplified sequence was the same as 99% as publication,with which replaced previous promoter CMV cloned in pEGFP c 1 expressional vector and transfected the cultured Mammary cell in vivo .Through observation under UV microscope,it showed that GFP had been successfully expressed in Mammary Gland cell.Then,the cloned sequence can be served for the study of mammary gland expression construct.

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What this paper is about

′flanking region of goat BLG was amplified from goat genomic DNA according to the published whole sequence of goat BLG and cloned to pMD 18t vector correspondently.By sequencing,the amplified sequence was the same as 99% as publication,with which replaced previous promoter CMV cloned in pEGFP c 1 expressional vector and transfected the cultured Mammary cell in vivo .Through observation under UV microscope,it showed that GFP had been successfully expressed in Mammary Gland cell.Then,the cloned sequence can be served for the study of mammary gland expression construct.

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Available abstract

′flanking region of goat BLG was amplified from goat genomic DNA according to the published whole sequence of goat BLG and cloned to pMD 18t vector correspondently.By sequencing,the amplified sequence was the same as 99% as publication,with which replaced previous promoter CMV cloned in pEGFP c 1 expressional vector and transfected the cultured Mammary cell in vivo .Through observation under UV microscope,it showed that GFP had been successfully expressed in Mammary Gland cell.Then,the cloned sequence can be served for the study of mammary gland expression construct.

Key concepts: Cloning (programming), Transfection, Biology, Molecular biology, Green fluorescent protein, Sequence (biology), DNA sequencing, Cloning vector

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