Simultaneous determination of vanillin,ethyl-vanillin,maltol and ethylmaltol in cigarette additives by high performance liquid chromatography

High performance liquid chromatographic method for simultaneous determination of vanillin,ethylvanillin,maltol and ethylmaltol in cigarette additives was established.After diluted,vibration and filtration,the cigarette additives were separated on a Thermo Scientific C18(4.6 i.d × 250 mm,5 μm) column in isocratic elution mode using acetonitrile:0.1% formic acid(33:67,V /V) as the mobile phase at 1.0 mL /min.The quantification of these cigarette additives was achieved with external standard method on a diode- array detector at 273 nm.Baseline separation of vanillin,ethyl-vanillin,maltol and ethylmaltol was achieved within 9 min.The linear ranges for vanillin,ethyl-vanillin,maltol and ethylmaltol were 0.0139 ~ 264.5 mg /kg,0.0554 ~302.7 mg /kg,4.01 ~ 262.9 mg /kg and 2.32 ~ 258.1 mg /kg,respectively.The linear correlation coefficients for vanillin,ethyl-vanillin,maltol and ethylmaltol were 0.9998,0.9999,0.9999 and 0.9997,respectively.The detection limits(S /N = 3) for vanillin,ethyl-vanillin,maltol and ethylmaltol were 0.00349,0.00554,1.29,and 0.58 mg /kg,respectively.The recoveries and the relative standard deviations(RSD,n = 9) at the spiked concentration of 10.75- 59.14 mg /kg were 96.8%- 102.2% and 0.62%- 1.6%,respectively.The proposed method has the merits of easy operation,short analysis time and accurate results,which is very suitable for the simultaneous determination of vanillin,ethyl-vanillin,maltol and ethylmaltol in cigarette additives.