Determination of Organophosphrous Pesticides Residue in Tea Using ASE Extraction-GPC/SPE Purification GC-MS Analysis

A method for determination organophosphrous pesticides residues in tea sample was described by accelerated solvent extraction-gel permeation chromatography/solid phase extraction purification gas chromatography-mass spectrometr.The target anslytes were extracted with the solution of acetone/dichloromethane(1∶1) using accelerated solvent extraction(ASE),then purified using gel permeation chromatography(GPC) and Carb/NH2 solid phase extraction.The separation and determination of analytes were performed by using GC-FPD and GC-MS with selected ion monitoring(SIM) mode and external standard method.Under the optimal conditions,the detection limits(S/N=3) of pesticides were in the range of 0.001 mg/kg-0.007 5 mg/kg with the relative standard deviations of 2.4 %-8.1 %.The recoveries of pesticides from teas sample spiked with mixed standards at 0.050 mg/kg ranged from 76.3 %-94.6 %.The method was rapid,precise,sensitive,high efficient in extraction convenient and purification.After routine applications,the results indicated that this method is suitable for the determination of organophosphrous pesicide resdues in the tea.