Quality Evaluation of Propolis Contents in Different Regions

Objective:To establish the methods of Propolis content determination from different regions.Methods:Thin layer chromatography(TLC) was adopted,using chrysin and galangin as reference substance and toluene,ethyl acetate-formic acid(10 ∶ 4∶ 3) as developing agent,to study propolis of different regions.High performance liquid chromatography(HPLC) method was adopted,using octadecyl silane bonded silica as stationary phase.Methanol 0.15% phosphoric acid aqueous solution(62∶ 38) was as mobile phase;setting flow rate was 1.0 ml / min,detection wavelength was 268 nm,to determine the contents of chrysin and galangin in propolis from different regions.Results:There were only six areas where the galangin and chrysin met the standards given by Pharmacopoeia of China(2010 version) from 28 samples of different regions.Conclusion:Contents of chrysin and galangin from propolis of different origins vary.The established quantitative and qualitative methods are accurate,reliable and exclusive,providing a basis for the quality control of propolis and its pharmaceutical preparation.