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[Quantitative analysis of galangin from propolis in different areas by HPLC].

Xiaoping Wang, Lin Li, Zhen-Lin Chen, Yanyan Ma, Jian-Guo Pan

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Abstract

OBJECTIVE: To establish an HPLC method for determination of galangin in propolis. METHODS: HPLC method was established. Diamonsil C18 column was used, CH3OH-4% H3PO4 solution (65:35) as mobile phase, at the flow rate of 1.0 ml/min and the UV detection wavelength at 256 nm. RESULTS: The linear of galangin was 0.00412 - 0.02472 mg/ml (r2 = 0. 9998). The content of galangin was the highest in the propolis from Shandong (11.7 mg/g). The content of galangin was higher in the propolis from Neimeng and Henan, respectively 10. 5 mg/g,10. 3 mg/g, the content of galangin was the lowest in the propolis from Gansu (9.8 mg/g). CONCLUSION: HPLC method is simple and reliable for determination of galangin in propolis.

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What this paper is about

OBJECTIVE: To establish an HPLC method for determination of galangin in propolis. METHODS: HPLC method was established. Diamonsil C18 column was used, CH3OH-4% H3PO4 solution (65:35) as mobile phase, at the flow rate of 1.0 ml/min and the UV detection wavelength at 256 nm. RESULTS: The linear of galangin was 0.00412 - 0.02472 mg/ml (r2 = 0. 9998). The content of galangin was the highest in the propolis from Shandong (11.7 mg/g). The content of galangin was higher in the propolis from Neimeng and Henan, respectively 10. 5 mg/g,10. 3 mg/g, the content of galangin was the lowest in the propolis from Gansu (9.8 mg/g). CONCLUSION: HPLC method is simple and reliable for determination of galangin in propolis.

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Available abstract

OBJECTIVE: To establish an HPLC method for determination of galangin in propolis. METHODS: HPLC method was established. Diamonsil C18 column was used, CH3OH-4% H3PO4 solution (65:35) as mobile phase, at the flow rate of 1.0 ml/min and the UV detection wavelength at 256 nm. RESULTS: The linear of galangin was 0.00412 - 0.02472 mg/ml (r2 = 0. 9998). The content of galangin was the highest in the propolis from Shandong (11.7 mg/g). The content of galangin was higher in the propolis from Neimeng and Henan, respectively 10. 5 mg/g,10. 3 mg/g, the content of galangin was the lowest in the propolis from Gansu (9.8 mg/g). CONCLUSION: HPLC method is simple and reliable for determination of galangin in propolis.

Key concepts: Galangin, Propolis, Chromatography, High-performance liquid chromatography, Chemistry, Food science, Flavonoid, Biochemistry

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