Experimental Study on Effects of Human Placenta on Leptin in Rats with Nonalcoholic Fatty Liver Disease

Objective:To investigate the effects of human placenta to the rats with nonalcoholic fatty liver disease and its influence on the cytokines such as Leptin.Methods:Forty-two male Wistar rats were randomly divided into four groups:normal group、model group、Essentiale group 、human placenta high dose group and human placenta low dose group.After treated with different agentia already definited for four weeks,histological examinations of liver were carried out with HE staining;meanwhile,the serum and tissue levels of Leptin were detected with RIA.Results:Histological examinations showed that there were severe fatty degeneration of hepatocytes in the model group.The damage in livers with low dose human placenta extracts and Essentiale was slightly milder than that of the models(P 0.05) .While high dose human placenta could obviously lessen the fatty degeneration(P 0.05) .Compared with the blank control group,the serum and tissue levels of Leptin in the model group were all remarkably increased(P 0.05 or P 0.01) .And the human placenta and Essentiale could significantly lower the levels of Leptin(P 0.05 or P 0.01) .Conclusion:Human placenta is effective in treating fatty liver induced by high-fatty diet .This may be due to its effects in lowering the levels of the Leptin which were concerned with the pathogenesis of nonalcoholic fatty liver disease.