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2004•Chinese Journal of Applied ChemistryRequires access

Extraction,Separation, HPLC Analysis of the Main Anthraquinones in Polygonum cuspidatum

Yu Lu

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Abstract

The extraction and separation processes of the main anthraquinones in Polygonum Cuspidatum, including emodin, rhein and physcion, have been carried out and improved. More pure emodin products without physcion could be obtained from a small amount and by repetitive extraction and stepwise collection. The extraction yields of emodin and physcion were more than 70%, with purity greater than 96%. The HPLC analysis method of anthraquinones was improved by using C18 column instead of C8 column, with detection wave-length of 437 nm. The method could afford to simutanniously determine the main anthraquinones in Polygonum Cuspidatum.

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What this paper is about

The extraction and separation processes of the main anthraquinones in Polygonum Cuspidatum, including emodin, rhein and physcion, have been carried out and improved. More pure emodin products without physcion could be obtained from a small amount and by repetitive extraction and stepwise collection. The extraction yields of emodin and physcion were more than 70%, with purity greater than 96%. The HPLC analysis method of anthraquinones was improved by using C18 column instead of C8 column, with detection wave-length of 437 nm. The method could afford to simutanniously determine the main anthraquinones in Polygonum Cuspidatum.

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Available abstract

The extraction and separation processes of the main anthraquinones in Polygonum Cuspidatum, including emodin, rhein and physcion, have been carried out and improved. More pure emodin products without physcion could be obtained from a small amount and by repetitive extraction and stepwise collection. The extraction yields of emodin and physcion were more than 70%, with purity greater than 96%. The HPLC analysis method of anthraquinones was improved by using C18 column instead of C8 column, with detection wave-length of 437 nm. The method could afford to simutanniously determine the main anthraquinones in Polygonum Cuspidatum.

Key concepts: Emodin, Anthraquinones, Chemistry, Chromatography, Polygonum, Extraction (chemistry), High-performance liquid chromatography, Anthraquinone

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