Screening and Identification of Antagonistic Bacteria Strains against Fusarium oxysporum f.sp.niveum
Zhiyi Chen
Abstract
Zhiyi Chen
Abstract
In this paper,1050 bacterial strains were isolated from the rhizosphere soil and roots of watermelon,cucumber,and eggplant in Inner Mongolia,Xinjiang,Anhui,Henan,Jiangsu and Jiangxi province.Among the tested strains,9 strains could inhibit mycelia growth of Fusarium oxysporum f.sp.niveum,Alternaria brassicicola,Phytophthora capsici and Botrytis cinerea.Two strains,NBT-15 and PL-82,had strong inhibitory activity against F.oxysporum,with inhibition rate of 60.5% and 55.3%,respectively.In pot experiment,control efficiency of NBT-15 and PL-82 against F.oxysporum after 30 days' treatment were 80.4% and 75.6%,respectively.Based on their morphology,physiological and biochemical characteristics and 16S rDNA sequence,the strains NBT-15 and pL-82 were identified as Bacillus subtilis and Pseudomonas aeruginosa,respectively.
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In this paper,1050 bacterial strains were isolated from the rhizosphere soil and roots of watermelon,cucumber,and eggplant in Inner Mongolia,Xinjiang,Anhui,Henan,Jiangsu and Jiangxi province.Among the tested strains,9 strains could inhibit mycelia growth of Fusarium oxysporum f.sp.niveum,Alternaria brassicicola,Phytophthora capsici and Botrytis cinerea.Two strains,NBT-15 and PL-82,had strong inhibitory activity against F.oxysporum,with inhibition rate of 60.5% and 55.3%,respectively.In pot experiment,control efficiency of NBT-15 and PL-82 against F.oxysporum after 30 days' treatment were 80.4% and 75.6%,respectively.Based on their morphology,physiological and biochemical characteristics and 16S rDNA sequence,the strains NBT-15 and pL-82 were identified as Bacillus subtilis and Pseudomonas aeruginosa,respectively.
Key concepts: Fusarium oxysporum, Biology, Rhizosphere, Botrytis cinerea, Antagonism, Mycelium, Bacillus subtilis, Fusarium wilt