Molecular Cloning and Sequence Analysis of the Amino Acid Transporter b~(0,+)AT-2 of Pigs

The cloning,sequencing of porcine b0,+AT transcript variant 2 was described in this study.Based on the sequences of human and mouse b0,+AT published on the NCBI,we identified a partial cDNA using the primers designed after bioinformatics analysis.Using rapid amplification of cDNA ends(RACE),the full-length cDNA encoding porcine b0,+AT-2 was sequenced.The porcine b0,+AT-2 cDNA was 1 488 bp long,including 90 bp 3′UTR and 126 bp 5′UTR,and CDS encoded 423 amino acids.The b0,+AT-2 protein sequence was found to be 75.1%,71.9% and 86.6% identities with human b0,+AT,mouse b0,+AT and swine b0,+AT-1.