2006•Zhongguo shouyi kexueRequires access
Molecular cloning and sequence analysis of interferon gamma gene from ten local breeds of chickens in Guangxi
Jianhua Sun
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Abstract
A pair of specific primers was designed according to the sequence of chicken interferon-gam-ma gene (ChIFN-γ) published in GenBank. The ChIFN-γ gene was amplified by RT-PCR from chickens' spleen lymphocytes of ten local breeds, which were activated by concanavalin A(ConA). After being cloned into the pMD18-T vector, the nucleotide acid sequences of ChIFN-γ gene were identified by PCR and digestion with restrictive endonucleases. Results showed that the coding region of ChIFN-γ gene encoded 145 amino acids, with a predicted mature protein of 16. 8 ku. Compared with that of mammals and other chickens, the sequence homology were 38. 8%-39. 0% and 99. 2%-100% in nucleotide acids, respectively, and 23. 6%-24. 8% and 97. 6%-99. 4% in amino acids, respectively.