Induction of apoptosis of human colon cancer HT-29 cells by 5,7-Dihydroxy-8-nitrochrysin

ObjectiveTo investigate induction of apoptosis of human colon cancer HT-29 cells by 5,7-dihydrox-8-nitrochrysin in vitro. MethodsThe proliferative activity of HT-29 cells treated with 5,7-dihydrox-8-nitrochrysin was measured using 3-(4,5-dimethylthiazol-2-yl) -2,5-dipheny tertrazolium blue(MTT) colorimetric assay. HT-29 cell apoptotic percentage was detected by flow cytometry with PI staining. DNA ladder bands were observed by DNA agarose gel electrophoresis. ResultsMTT assay suggested that 5,7-dihydrox-8-nitrochrysin inhibited proliferation of HT-29 cells in a concentration-dependent manner,and IC50 was 1.78 μM. FCM with PI staining and DNA agarose gel electrophoresis indicated the induction of apoptosis on HT-29 cell line treated with 5,7-dihydrox-8-nitrochrysin.The pre-incubation of GW9662,blocker of PPARγ,can antagonize the induction of apoptosis of HT-29 cells by 5,7-dihydrox-8-nitrochrysin. Conclusion5,7-dihydrox-8-nitrochrysin induces apoptosis of HT-29 cell line by activating PPARγ.