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Spectrophotometric Method for the Determination of Proteins with TZACA

Deman Han

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Abstract

2-(2,3,5-triazolylazo)-1,8 dihydroxynaphthale-3,6-disulfonic acid reacts with proteins to form complexes in ClCH_2COOH-CH_3COONa buffer with pH 2.96. The maximum absorption wavelength was at 505.80 nm. The optimum reaction condition was investigated and a new method for the determination of protein was developed based on this binding reaction. Beer's law was obeyed in the range from 0 to 80 mg·L-1. The proposed method , which is rapid ,stable and of good simple selectivity and high sensitivity, has been applied to the spectrophotometric determination of total proteins in human serum samples with satisfactory results.

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What this paper is about

2-(2,3,5-triazolylazo)-1,8 dihydroxynaphthale-3,6-disulfonic acid reacts with proteins to form complexes in ClCH_2COOH-CH_3COONa buffer with pH 2.96. The maximum absorption wavelength was at 505.80 nm. The optimum reaction condition was investigated and a new method for the determination of protein was developed based on this binding reaction. Beer's law was obeyed in the range from 0 to 80 mg·L-1. The proposed method , which is rapid ,stable and of good simple selectivity and high sensitivity, has been applied to the spectrophotometric determination of total proteins in human serum samples with satisfactory results.

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Available abstract

2-(2,3,5-triazolylazo)-1,8 dihydroxynaphthale-3,6-disulfonic acid reacts with proteins to form complexes in ClCH_2COOH-CH_3COONa buffer with pH 2.96. The maximum absorption wavelength was at 505.80 nm. The optimum reaction condition was investigated and a new method for the determination of protein was developed based on this binding reaction. Beer's law was obeyed in the range from 0 to 80 mg·L-1. The proposed method , which is rapid ,stable and of good simple selectivity and high sensitivity, has been applied to the spectrophotometric determination of total proteins in human serum samples with satisfactory results.

Key concepts: Chemistry, Chromatography, Buffer solution, Selectivity, Linear range, Absorption (acoustics), Spectrophotometry, Analytical Chemistry (journal)

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