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Analysis of SSR Markers of Erucic Acid Content in Brassica Napus

Chen She-yuan

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Abstract

Objective: This research was studied to promote the development high erucic acid rape breeding. Method: F2 groups which were hybridized by High erucic acid material and low erucic acid hybrid material were studied here, and the parents' erucic acid content was analyzed by SSR markers. Results: The erucic acid content was controlled by two genes, and the inheritance rule accorded with the segregation ratio which is controlled by one pair gene. Meanwhile, two codominant markers of CB10364 and Ra2-E12 were ob- tained. Conclusion: CB10364 markers tightly linked with the erucic acid content. Individual plant strip type of CB10364-a has the erucic acid content 6%, while the type of CB10364-h has the erucic acid content from 6% to 36%, and that of CB10364-b has the erucic acid content 36%. The results can be used on high erucic acid rapeseed breeding, and may be highly availability.

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What this paper is about

Objective: This research was studied to promote the development high erucic acid rape breeding. Method: F2 groups which were hybridized by High erucic acid material and low erucic acid hybrid material were studied here, and the parents' erucic acid content was analyzed by SSR markers. Results: The erucic acid content was controlled by two genes, and the inheritance rule accorded with the segregation ratio which is controlled by one pair gene. Meanwhile, two codominant markers of CB10364 and Ra2-E12 were ob- tained. Conclusion: CB10364 markers tightly linked with the erucic acid content. Individual plant strip type of CB10364-a has the erucic acid content 6%, while the type of CB10364-h has the erucic acid content from 6% to 36%, and that of CB10364-b has the erucic acid content 36%. The results can be used on high erucic acid rapeseed breeding, and may be highly availability.

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Available abstract

Objective: This research was studied to promote the development high erucic acid rape breeding. Method: F2 groups which were hybridized by High erucic acid material and low erucic acid hybrid material were studied here, and the parents' erucic acid content was analyzed by SSR markers. Results: The erucic acid content was controlled by two genes, and the inheritance rule accorded with the segregation ratio which is controlled by one pair gene. Meanwhile, two codominant markers of CB10364 and Ra2-E12 were ob- tained. Conclusion: CB10364 markers tightly linked with the erucic acid content. Individual plant strip type of CB10364-a has the erucic acid content 6%, while the type of CB10364-h has the erucic acid content from 6% to 36%, and that of CB10364-b has the erucic acid content 36%. The results can be used on high erucic acid rapeseed breeding, and may be highly availability.

Key concepts: Erucic acid, Rapeseed, Brassica, Biology, Food science, Agronomy

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