Effect of Red Clover Isoflavones on Liver Function and Inflammatory Response of Nonalcoholic Fatty Liver Disease in Rats

Observation of Red Clover Isoflavones on liver function and inflammatory response of nonalcoholic fatty liver disease in rats. After one week feeding in laboratory, 12 out of 54 male SD rats were randomly chosen as the normal control group and fed with normal diet. The rest were made as modules, gavage feeding with fat emulsion and 18 % sucrose solution. Two rats from normal control group and two from module were killed randomly after 4 weeks. Liver biopsy showed the module formation was successful. Then we randomly divided module into 4 groups(n=10) according to weight: Nonalcoholic fatty liver diseases(NAFLD) model control group, high-dose, medium-dose and low-dose Red Clover Isoflavones treatment group(75, 150, and 300 mg/kg),respectively. The experiment period lasted 12 weeks. At the end of experiment, rats were killed to collect serum and liver tissues. Then we detected the activity and content of Aspartate aminotransferase(AST), alanine aminotransferase(ALT), total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDLC), high density lipoprotein cholesterol(HDL-C), malondialdehyde(MDA), superoxide dismutase(SOD),alanine aminotransferase(ALT), aspartate aminotransferase(AST) and free fatty acids(FFA). Compared with model group, the middle-dose group and the high-dose group could significantly decrease TC, TG, LDL-C and FFA levels in serum and liver tissues and increase HDL-C levels. Compared with the model group, the middle-dose group and the high-dose group of red clover isoflavones could decrease MDA levels and improve SOD activity in serum, and the differences were statistically significant(p0.05). The levels of ALT and AST(145.88±34.28) in middle and high dose group were lower than control group(p0.05). Moderate dose of Red Clover soflavones could regulate lipid metabolism and reduce oxidative damage caused by high sugar and fat diet. Besides, the injury of liver cells was relieved and liver function was improved. For rats with nonalcoholic fatty liver disease, Red Clover Isoflavones had some certain protective effect on their.