Effect of phytohormone on regeneration system of two alfalfa varieties

The regenerative systems of alfalfa varieties(Millennium and Gannon No.4)were comparatively studied by using MS as basic medium adding different concentration of 2,4-D,KT and NAA.The results showed that the hypocotyl was the optimal explant material;2,4-D level had the less effect to callus induction of Gannon No.4,but evidently enhanced Millennium with the increasing concentration of 2,4-D.The effective media for inducing callus of these two variety was MS containing 2 mg/L 2, 4-D ,0.3 mg/L KT,30 g/L sucrose and 7.5g/L agar.The optimal differentiation medium was MS containing 0.5 mg/L KT,0.1 mg/L NAA and 20 g/L sucrose.The effective root inducing medium was 1/2 MS containing NAA0.5 mg/L.