Determination of the Blood Concentration of Mycophenolate Mofetil in Lupus Nephritis Patients by HPLC

OBJECTIVE:To determine the blood concentration of mycophenolate mofetil(MMF)in the lupus nephritis(LN)patients. METHODS:HPLC was used for determining MMF metabolite mycophenolic acid(MPA)after blood sample of LN patients trated. SGE Protecol C18 column was used with mobile phase consisted of methanol-acetonitrile0.01% phosphoric acid(23 ∶ 37 ∶ 40,V/V/V)at the flow rate of 1.0 ml/min. The detection wavelength was set at 216 nm,and injection volume was 20 μl. The column temperature was 30 ℃. RESULTS:The linear range of MPA was 0.125-10 mg/L(r=0.999 9)with method recovery of 98.67%-104.31% and extraction recovery of 83.43% -97.54% . The extraction recovery of internal standard(nimesulide)was 79.35% . RSDs of inter-day and intra-day were 2.6%-7.7% and 2.3%-8.5%,respectively. CONCLUSIONS:The method is simple and accurate,and can be used for blood concentrations monitoring of MPA in LN patients after taking MMF.