Fermentation and Extraction of Polysaccharides from Coriolus versicolor and Its Antioxidant Activity

By using single factor experiment and central composite design,the optimal culture conditions of Coriolus versicolor for polysaccharides production were determined as polyptone 5.870 g/L,maltose53.120 g/L,pH 5,temperature 28℃ shear speed 150 r/min.Under these conditions,the EPS production reached at 10.0982 g/L.Furthermore,EPS were treated by Sevag method to remove the protein and purified by gel filtration on Sepharose CL-6B chromatography.The antioxidant activity was determined by Phenanthroline-Fe2+ method and DPPH assay.The polysaccharides showed notable inhibitory activity of OH and DPPH radical.Thus,the EPS from C.versicolor may be supplied as one natural healthy food.