Study of the Different Ratio of Angelica sinensis and Astragalus membranaceus injection on Angiogenesis of Chick Embryo Chorioallantoic Membrane Model

Objective: To investigate the effect of the different ratio of Angelica sinensis Injection(ASI) and Astragalus membranaceus Injection(AMI) on angiogenesis of the chick embryo chorioallantoic membrane(CAM).Methods: After fertilized eggs were incubated for 7 days at(37.8 ℃,) the chorioallantoic membrane(CAM)models were made,and were respectively treated with NS(negative control),different ratio of ASI and AMI,and EGF(positive control).After incubated for another 3 days,the CAM samples were made,then counted the number of the first grade and second grade blood vessels(capillaries).Result: The proliferation of capillaries in the therapy groups was obviously increased compared with NS control(P0.05),but the effect is lower than EGF control(P0.05) excluding the group of ASI∶AMI=1∶5.Conclusion: The result showed that the combination of different ratio of ASI and AMI can obviously stimulate angiogenesis of the chick embryo CAM.And proliferation and migration of vascular endothelial cells can be the mechanism of angiogenesis.